Sentence examples for based on luciferase fragment from inspiring English sources

Toward a simple, sensitive and rapid method for measuring IP3, we describe the development and application of a novel biosensor based on luciferase fragment assisted complementation strategy, which converts the ligand-induced conformational changes to light.

Unfortunately, this level of labeling also skewed viral activity based on luciferase activity and qPCR of viral genomes.

These results indicate that during the cytolysis process, luciferase does not readily release to the outside and thus the supernatant is not the ideal source for monitoring cell viability based on luciferase assay.

Nevertheless, based on luciferase reporter gene assays it has been suggested that PPARα-tr may exert dominant-negative functions [ 9].

Successful crosses were identified based on luciferase imaging in the F1 generation and plants were allowed to self-pollinate.

The recombinant Ad5 genomes were based on luciferase (Luc -expressing repLuc -expressingent (ΔE1/ΔE3) Ad5 (referred to as Ad5.Luc throughout the article).

The subsequent development of a plasmid based on luciferase activity instead of radioactivity resulted in stable transfection efficiencies [ 38].

Trapping is not a general problem with PCAs and in particular, we have demonstrated that new classes of PCAs based on luciferases are completely reversible; that is, dissociation of protein complexes leads equally to unfolding and physical separation of the complementary reporter protein fragments [30], [31].

Alternative experimental approaches using reporter assays based on luciferases or fluorescent proteins have also been developed.

Recently, Frank's group published a study on the GH receptor activation process based on complementation of luciferase fragments which concluded that GH binding increases complementation i.e. brings the cytoplasmic domains closer together [ 60].

Firefly luciferase activity was normalized based on Renilla luciferase activity.