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Basic bioinformatic approaches designed to predict protein localization are based on local sequence pattern at the presumed location of targeting signals, mainly within the N or C-terminus.
In those places a single template was used based on local sequence similarity with ASP-2A.
The performance of programs that are based on local sequence similarities, on the other hand, is systematically underestimated by BAliBASE.
To detect sequence similarity, most methods use BLAST, a fast heuristic algorithm for identifying homology between sequences based on local sequence alignments.
The two repressive marks can also be readily distinguished from each other based on local sequence, which again is consistent with existing experimental data on a broad scale [ 34].
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Mismatch rates were also incorporated in the variant detection model to dynamically adjust error rates based on local sequencing errors.
Based on local and global sequence identity, the sequences of our engineered mAbs are indistinguishable from those of fully human mAbs isolated from transgenic mice or human antibody libraries.
The KEGG resource [ 56] uses a novel approach to cluster proteins into orthologous groups that is distinct from both phylogenetic and graph-based approaches: new sequences are included based on local similarity to sequences already in a KEGG orthology group.
Our method is based on local alignments and discontiguous sequence similarity.
Second, from the non-coding regions of every group of orthologous genes, we selected several 1 kb long "orthologous" regions based on local alignments and discontiguous sequence similarity (See Methods).
Robetta server [ 104] was used to generate the peptide fragments based on local homology of the ycf39 sequence with other sequences in a structural database.
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