The volume decrease between prophase and metaphase is consistent with a recent study [3], also based on live cell imaging, where it was proposed that a "pre-mitotic condensation" process marks a clear reduction in cell volume before entering into mitosis.
Although we have access to AF cells with other trisomies, we would like to point out that the experiments we performed (including several based on live-cell imaging) are technically very challenging in amniocytes.
Based on our live-imaging analysis of dsh and dsh Vang mutant SOPs, we postulate the existence of at least one effector acting downstream of Vang that regulates spindle orientation.
In addition, the experiments, which are largely based upon live imaging approaches, are carefully performed and analyzed.
Based on in-vivo live imaging and DAPI analysis, samples of cultured microspores, multicellular pro-embryos and embryo at different developmental stages from different experimental treatments, were fixed in 4% paraformaldehyde in PBS, overnight, at 4°C.
As expected based on the live cell imaging results, we saw an increase in the invasive capacity of MCF10A cells co-expressing CUX1 and GLIS1 compared to either one alone (Fig. 7G,H).
Tractography-based parcellation is a method based on diffusion imaging tractography, which segregates the living human brain into distinctive areas showing sharp differences in their anatomical connectivity.
Previous estimates of network structure, based on such imaging, have been sketchy.
Maybe one day in the far future we will be able to make psychiatric diagnoses based on brain imaging alone.
