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The quantitative assay of the minimal inhibitory concentration (MIC; μg/mL) was based on liquid medium twofold microdilutions performed in 96-multi-well plates.
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It settled on an idea based on liquid crystals.
Such devices are based on liquid crystals, materials that have a structure somewhere between that of a highly ordered solid and liquid where the molecules are disordered.
Dec/hyd ratios were determined based on liquid phase composition.
The NGMN method was based on liquid-liquid extraction followed by HPLC-MS/MS analysis.
Steroid determinations were based on liquid-liquid extractions and on solid-phase extractions with commercially available cartridges.
Each submitted sample was cultured on at least one liquid medium and three solid media.
Note that the use of liquid versus solid medium depended on the evolution of DST methods; thus, DST was performed on solid medium before 2007, and on liquid medium after this date.
In this work, coupling of effervescence-assisted dispersive liquid-liquid microextraction based on a medium-chain fatty acid (EA-DLLME-FA) with accurate micro-sampling flame atomic absorption spectrometric (MS-FAAS) analysis was developed for the first time as a swift, facile, and effective analytical procedure for the satisfactory analysis of Ag+ and Co2 + ions from different real samples.
Sample preparation was based on simple liquid-liquid extraction into cyclohexane (1 : 1).
This design was performed in two medium types: rich YM medium and minimal medium, based on date juice (Yucca medium).
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