Hybrid capture (HC2) is based on liquid phase hybridization using long synthetic RNA probes complementary to the genomic sequences of 13 high-risk types (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68) and five low-risk types (6, 11, 42, 43 and 44); these are used to prepare high-risk (B) and low-risk (A) probe cocktails that are used in two separate reactions 139.
And for both DNA samples (single cells and matched tissues), whole exome capture was accomplished based on liquid phase hybridization of sonicated 2 μg genomic DNA to the bait cRNA library synthesized on magnetic beads using the SureSelect® Human All Exon 50 Mb kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer's protocol.
Hybridization-based methods include northern blot hybridization and those based on liquid-phase hybridization, such as nuclease protection assay.
We describe here an HCV genotyping assay based on liquid microarray technology and including 16 hybridization probes specific for different HCV genotypes and subtypes and one set of primers for each of the two regions most frequently used for HCV detection and genotyping (5'UTR and NS5B).
It settled on an idea based on liquid crystals.
Dec/hyd ratios were determined based on liquid phase composition.
The NGMN method was based on liquid-liquid extraction followed by HPLC-MS/MS analysis.
Steroid determinations were based on liquid-liquid extractions and on solid-phase extractions with commercially available cartridges.
Although the conventional analytical methods used to extract APEO compounds from liquid samples were initially based on liquid-liquid extraction (LLE), researchers have recently favoured the replacement of LLE with SPE for liquid samples [ 152].
Because microarray is based on probe-target hybridization, cross hybridization among probes from members of gene families could produce false-positive signals under some hybridization conditions.
