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Dead cells were excluded based on light scatter characteristics and gates were set to select the brightest and dimmest 2 3% of the analyzed population on average.
To avoid debris, electronic background, and undesired clumps in the bacterial samples, a Gate R1 was set up, which was based on light scatter for the flow cytometry assay.
Analysis was based on light scatter, and fluorescence signals produced from laser illumination at 640 nm.
Cell doublets were excluded based on light scatter, except for DC enumeration.
10,000 events were acquired and gates were set based on light scatter properties to exclude debris and nonviable cells.
The differential cell count was processed according to Sladek et al. [ 25] based on light scatter properties (Fig. 1).
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For particles with sizes at the upper range of the Rayleigh regime and even into the Mie scattering regime (particle sizes comparable to the wavelength), sensors based on light scattering form a viable option for nanoparticle detection, characterization, and sizing.
Here, we report a high-throughput technique for screening buffer components to stabilize capsomeres, based on light scattering analysis.
An optical probe of the methods based on light scattering for measuring surface roughness and optical triangulation for measuring micro-displacement is described.
The basic algorithms for aerosol retrieval is based on light scattering simulations of polarization field, where the heterogeneous aerosol model according to Maxwell-Garnett mixing rule is considered.
Based on light scattering measurements, it has been shown that the polymerization of the polyvanadic acid precursor solutions does not stop even after 1 year.
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based on light arc
based on light oil
based on light manufacturing
based on light rail
based on light interference
based on light wave
based on light level
based on light use
based on light irradiation
based on light transport
based on light video
based on light scan
based on light distribution
based on light detection
based on light scattering
based on light weight
based on light absorption
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