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This claim was, however, based on light microscope morphology and expression of surface proteins alone.
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Before the advent of advanced imaging, an understanding of the pathology of gout was based primarily on light microscope examination of tophi and periarticular bone, supplemented by XR to define the radiographic morphology and distribution of erosions [ 7].
The microscope is based on light interferometry and operates as a noncontact optical profiler in vertical scanning interferometry mode to produce 3D topography maps of the sample surface.
Determine the shutter speed based on light.
Two aliquots of 100 μL each were counted on light microscope.
Based on these light microscopic investigations, ultrathin sections from selected tissue blocks were counterstained with uranyl acetate and lead citrate and examined with a Philips CM 10 transmission electron microscope.
The easy applicability of this bifocal lens is demonstrated on a realized microscope setup for volumetric observation of freely moving fluorescent objects, based on a conventional light microscope.
At last, the 24-well plates were observed by laser confocal microscope to compare the cellular uptake efficiency based on the light intensity of emission light.
To quantify cell size at 3 hours of adhesion, total cell number was determined based on coomassie staining under light microscope, total area occupied by cells was determined by the imaging software (NIS-Elements AR 2.30 software; Nikon France), and mean surface per cell was calculated (area/number of cells).
As described in Methods, our microscope is based on circularly polarized light, allowing contrast to be independent of crystal orientation within the plane perpendicular to the microscope's optical axis, and generating images in which intensity is proportional to birefringent retardance.
Here we report about such development of an FMS system based on a white light spinning disk confocal microscope.
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