Sentence examples for based on ligation of from inspiring English sources

Probe construction based on ligation of three smaller constructs [45] would allow for variability in fragment assembly.

In our studies, the detection was based on ligation of linear DNA to a compatible linker.

GoldenGate cloning is based on ligation of multiple inserts in sequential order through the action of type II restriction enzyme BsaI and of T4 DNA ligase in a single restriction-ligation reaction, followed by transformation into competent E. coli cells.

This protocol, based on ligation of adapters directly to the 3′ and 5′ ends of the RNA molecules, has the advantage of preserving the information about the transcript orientation.

MLPA is a technology based on ligation of multiple probe-pairs hybridized across the entire locus of interest, followed by amplification, facilitated through the use of universal-tag PCR primers for all ligated probe pairs, and subsequently fragment analysis.The use of universal PCR means that amplicons are generated with comparable efficiency and thus the method is semi-quantitative.

The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4, 5].

The novel CL imaging strategy based on ligation-mediated RCA synthesis of DNAzyme showed high fidelity in discriminating single-base mismatch and efficiently facilitated signal amplification for sensitive target DNA detection.

The initial reports based on ligations at cysteine (Fig. 5A) inspired the development of alternative techniques where other thiol-containing residues were used (Fig. 5B and C).

For detection of CHEK2 *1100delC, we developed an assay based on ligation-dependent amplification.

In this expanded method denoted as ATLAS (A-dvanced T-elomere L-ength A-nalysis in S. cerevisiae), we have introduced: 1) set of new primers annealing with high specificity to telomeric regions on five different chromosomes; 2) new approach for designing reverse telomere primers that is based on the ligation of an adaptor of a fixed size to telomeric ends.

Briefly, the method is based on the ligation of oligonucleotide adaptors.