Sentence examples for based on ligation dependent from inspiring English sources

The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4, 5].

For detection of CHEK2 *1100delC, we developed an assay based on ligation-dependent amplification.

Probe construction based on ligation of three smaller constructs [45] would allow for variability in fragment assembly.

In our studies, the detection was based on ligation of linear DNA to a compatible linker.

GoldenGate cloning is based on ligation of multiple inserts in sequential order through the action of type II restriction enzyme BsaI and of T4 DNA ligase in a single restriction-ligation reaction, followed by transformation into competent E. coli cells.

Briefly, this technique is based on a ligation-dependent probe amplification of 12 to 28 probes (depending on the kit used) encompassing the NF1 area together with 11 to 15 additional control probes (also depending on the kit used).

To address both of the issues, we applied a strategy based on multiplex ligation-dependent probe amplification (MLPA).

A further development of the RespiFinder-19 assay is the RespiFinder-SMART-22 assay, which is also based on the multiplex ligation-dependent probe amplification.

Moreover, using the BeeDoctor diagnostic tool [ 17] which is based on the multiplex ligation-dependent probe amplification technology, we were also able to screen in parallel for the negative-strand intermediate.

The RespiFinder-19 assay, which is based on the multiplex ligation-dependent probe amplification analyzed by capillary electrophoresis, detects 15 respiratory viruses and 4 bacteria in one reaction [ 20].

This technique is based on the ligation followed by PCR amplification of two adjacently hybridising oligonucleotides.