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Herein we reported the development of aptamer-based biosensors (aptasensors) based on label-free aptamers and gold nanoparticles (AuNPs) for detection of Escherichia coli (E. coli) O157 H7 and Salmonella typhimurium.
Herein, we reported the development of aptamer-based biosensors (aptasensors) based on label-free aptamers and AuNPs for the detection of E. coli O157 H7 and S. typhimurium, with the aim of establishing a preliminary method to evaluate the utility of aptamer-AuNPs assay for enteropathogenic bacteria.
The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy.
In our work, we have made theoretical analysis of a perfect optical biosensor using the 1D PC based on label-free optical sensing, which does not use fluorescence based-detection, where porous silicon interacts with biomolecule and reduces its refractive index by biomolecular reaction.
To overcome these difficulties we combined a direct functional assay based on electrophysiology with a direct binding assay based on label-free tryptophan fluorescence.
This final study attempts to investigate the 'objective' severity of a set of diseases by asking participants to judge the relative severity of a pair of disorders based on label-free disease descriptions.
In this study, we firstly developed the method of blood serum detection by using SERS based on label-free Au nanoparticles to diagnose the parotid gland tumors preoperatively.
The differential peptide abundance between daytime and nighttime for 173 peptides was assessed based on label-free integrated precursor ion intensity from chromatographically aligned spectra (i.e., SIEVE analysis).
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Therefore, the new fluorescent analysis system could be applied for the detection of Pb2+ based on the label-free DNA sequence capped CdS QDs.
A new SERS method was developed for the determination of DA over 2.5 500 μmol/L based on the label-free probe.
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