The Taiwanese isolates of P. noxius were dividable into long (type L), median (type M) and short (type S) groups based on ITS sequence length.
Results from this study showed that the isolates of P. noxius from Taiwan can be divided into type L, type M and type S based on ITS sequence length.
However, the ITS nucleotides showed 20 different kinds of variations including single nucleotide polymorphisms, deletion and insertion in ITS1 and ITS2, but none in 5.8 S. The Taiwanese isolates of P. noxius were dividable into long (type L), median (type M) and short (type S) groups based on ITS sequence length.
This study shows that P. capsulatum can be identified based on ITS sequence data alone.
Three pairs of specific primers were designed based on ITS sequence of A. ceylanicum and A. caninum and TPI gene of G. lamblia available in the GenBank.
Cosner, in her thesis [ 9], included an early version of a portion of the work described here, and Eddie, in his thesis [ 10] developed phylogenetic hypotheses based on ITS sequence data and morphology.
Based on ITS sequence data, Sha et al. [ 11] reported that L. racemosus, L. arenarius, L. ramosus and L. paboanus were nested into one group, while L. angustus, L. tiancshanicus, and L. karelinii were in another group.
Isolates and species were selected to represent taxa most relevant to European forestry that also represented the breadth of Phytophthora diversity defined according to clades based on ITS sequence analysis [ 2].
The DNA ploidy data of analysed individuals were also located on the phylogenetic tree (maximum parsimony strict consensus tree) of A. senegal based on ITS sequence data (Fig. 4) (Odee et al. 2012).
For the SIX2 effector, the software SIGNALP 4.0 does not predict a signal peptide, yet in our analysis it is still placed in the same cluster as the secreted SIX1, SIX6, SIX9 and SIX10 effectors based on its sequence-derived features.
For example, the F. oxysporum SIX2 effector for which the software SIGNALP 4.0 does not predict a signal peptide is still placed in the same cluster as the secreted SIX1, SIX6, SIX9 and SIX10 effectors based on its sequence-derived features.
