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It is based on isolation of genomic DNA isolated from test and reference cell populations, differential labeling with fluorescent dyes and cohybridization with a high-resolution DNA microarrays.
The diagnosis of meningitis was based on isolation of a bacterium in one cerebrospinal fluid culture, as reiterated by Mayhall et al. [2].
Current records of possible pathogens in African fermented foods have been based on isolation of pure cultures, followed by phenotypic identification of isolated microorganisms (Ejiofor et al. 1987; Enujiugha and Akanbi 2008; Nwagu et al. 2010; Ogueke and Aririatu 2004; Okorie and Olasupo 2013a).
The mentioned method was based on isolation of Trichostrongylus ova from human fecal samples using Willis method, the extraction of ova genomic DNA samples, followed by rDNA ITSII PCR and one-step multiplex RE-PCR using three restriction enzymes of HinfI, DraI, and MseI.
To date, attempts to circumvent this difficulty were based on isolation of the different cell structures constituting biological samples.
A diagnosis of leptospirosis was based on isolation of Leptospira from blood and/or a positive MAT, which was defined as a 4-fold rise in titer between acute and convalescence samples or a single titer of ≥1∶400.
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Thus, the most widely used approaches to study the contribution of repetitive DNA to genome evolution are based on isolation and characterization of only a single or a small group of elements.
Will it be based on isolation, the cost of delivering services, or the distance to employment?
The extent to which generalized treatment recommendations can be based solely on isolation of so-called SPICE-MP (Serratia, Proteus spp. – Indole positive, Citrobacter, Enterobacter, Morganella, and Providencia) organisms, the 'usual suspects' for ampC carriage, remains limited [ 22, 23].
We propose that lactose synthesis and mammary gland involution does not normally occur in fur seal mammary glands due to absence of LALBA in fur seal milk and show that previous predictions based solely on isolation of cDNA in otariid mammary glands [ 11] are incorrect.
Diagnosis of A. galli is based on faecal isolation of parasite eggs or direct identification of adult worms in the intestine [ 21].
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