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These newly evolved genes may acquire introns in the untranslated regions by capture of nearby exons into a new genomic environment or fusion with host genes, which is chimerization based on intron gain [ 3- 8].
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This proportion (80 %) is significantly higher than the percentage of frameshift introns generated by chimerization based on intron-gain retrogenes (29.8 %, 16/49) (P-value = 0.017) [ 8].
Gene-based markers were designed based on intron sequences.
The non-Solanaceae donor of the Hyoscyameae/ Mandragora intron type is unclear based on intron phylogeny.
We calculated intron positions in the unigene based on intron positions in the Arabidopsis CDS.
Classes based on intron structure differed significantly in predicted chemistry.
Gene compactness was described based on intron number and average length of UTR/intron for a given gene.
Because only a few species were found to possess an intron 714 or an intron 870, our co-speciation analyses were only based on introns 723.
Debates on intron gain and loss as well as other evolutionary features are still ongoing [9], [10], [11], [12].
A few inferences regarding geographically structured populations of D. pulex can be extracted from our observations on intron gain.
This being said, there are indications of recent intron gain in some lineages, and the reference to Coghlan and Wolfe (Ref. 53) on intron gain in nematodes was added in support of this point.
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