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To enable comparison of our molecular clock calibration based on intraspecific variation with other estimates, the time represented by the scale bar in figure 3 was determined using other calibration methods.
We then used reserve selection software to prioritize areas for conservation based on intraspecific variation or species-level diversity.
This result suggests that the reverse is also true: reserves based on intraspecific variation do not adequately capture species-level variation.
Nevertheless, we are confident in concluding that reserves based on species-level data are not concordant with those based on intraspecific variation and that there is a need for both in conservation prioritization.
However, as the previous studies [ 17, 38, 39] are based on intraspecific variation it is understandable that the levels from the current study are much higher - 53 SNPs/kbp in Salix and 30 in Populus in the current study.
In contrast, the selective values S on synonymous codon usage we estimate using ROC SEMPPR are substantially smaller than those estimated by Lawrie et al. (2013) based on intraspecific variation in 4-fold degenerate sites for Drosophila melanogaster.
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We also demonstrate that priority areas for conservation-based species-level data are not concordant with those based on intraspecific genetic and morphological variation.
The three loci were chosen based on known intraspecific variation in flamingos (COI in Chilean and Greater Flamingos; NADH 2 in Lesser Flamingos) or known high rates of mutation (control region).
This study focused on global comparisons of traits across species and not on intraspecific variation.
Research on intraspecific variation in ciliates is scarce, and in scuticociliate parasite of fish, virtually nonexistent.
Here we review the current data on intraspecific variation in recombination rate and discuss the molecular and evolutionary costs and benefits of recombination frequency.
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