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Based on internal transcribed spacer regions, oligonucleotides were developed for seven operational taxonomic units (OTUs; defined as groups of sequences sharing at least 97% sequence similarity), corresponding to members of the Tulasnellaceae family.
This study reports a multiplex polymerase chain reaction (PCR) assay based on internal transcribed spacer-1 (ITS-1) for the simultaneous diagnosis of the Eimeria tenella, Eimeria acervulina, Eimeria maxima, and Eimeria necatrix species, which infect domestic fowl.
In recent years, this diverse group of organisms in the Northeast US has been classified into three distinct taxa (a Ribes-alternating group, a Larix-alternating group - M. paradoxa, and an Abies-alternating group M. americana) based on internal transcribed spacer (ITS) and partial large subunit (LSU) rDNA sequence phylogenetic analysis.
Phylogenetic analysis of European specimens, based on internal transcribed spacer and 5.8S DNA sequences, indicates that H. repandum specimens form two distinct clades, whose only consistent morphological distinction is cap size.
Lactarius vinosus has often been considered as a variety of L. sanguifluus, but morphological (especially macroscopic characters and spore-ornamentation) and molecular evidence (based on internal transcribed spacer-sequencing) has confirmed that they are separate species.
Morphological revision of the type and DNA sequence data (based on Internal transcribed spacer sequences) obtained from the collection confirmed that this taxon belongs in the genus Volvopluteus, and that it is a separate species from all the other members of that genus.
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Reason is based on internal measurements.
How they evolve based on internal and external stimuli.
The program is based on internal research conducted by REI.
The observations were based on internal IBM documents from 2017.
The operations approach is based on internal controls and an internal perspective.
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