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b) Adding a new fluorophore based on intensity distribution (Op_b).
In contrast, if the probability of Op_b is high, FID3B tends to select more fluorophores based on intensity distribution.
When model selection (Step 3) is executed to adjust the model to fit the data, we take one fluorophore under consideration from three operations: adding a new fluorophore at a random position (Op_a), adding a new fluorophore based on intensity distribution (Op_b) and randomly selecting a fluorophore in the model for removal (Op_c).
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Our method was based on intensity distributions for each marker that we computed [ 23] using our reference samples.
Therefore, we adaptively set its distribution based on intensity gradients for each area in the HR frame.
Open image in new window Figure 8 Particle size distribution patterns (based on intensity) obtained with BCGO powder prepared by chemical precipitation.
Open image in new window Figure 10 Particle size distribution patterns (based on intensity) obtained with BCSO powder prepared by chemical precipitation.
Size-distribution measurements based on intensity were carried out by DLS (dynamic light scattering) using a Zetasizer Nano-S instrument (Malvern Instruments) at 22 °C, with a count rate of 260 per s and a measurement duration of 80 s.
We proposed a simple overlap factor calculation method based on laser intensity distribution (OFLID), which is simple, practical and can be applied to any specific laser intensity distribution.
The key techniques include two aspects: an intensity distribution calculation to obtain the probability distribution of fluorophores and a modified model to add a procedure that selects the initial positions of fluorophores based on the intensity distribution.
The key techniques include two aspects: an intensity distribution calculation to obtain the probability distribution of fluorophores and a modified model selection to add a procedure that selects the initial positions of fluorophores based on the intensity distribution.
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