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We include a training set for these algorithms based on individual genotypes for 705 samples.
Genotypes of B. rapa were also well separated from those of B. napus in an UPGMA-tree based on individual genotypes.
A range of statistics that are based on individual genotypes rather than population level allele frequencies are now available [ 6, 7], which allow the assignment or exclusion of genotypes within sampled populations and hence provide a more ecologically relevant measure of connectivity [ 8, 9].
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The costs of GWAS projects based on individual genotyping are still comparatively high and increase with the size of study populations.
Most importantly, these estimates, and especially their averages across the pools, are highly similar to the allele frequencies based on individual genotyping.
The costs of projects based on individual genotyping using microarrays are still high and increase directly with the number of samples to be analyzed.
We used two Bayesian methods implemented in the programs STRUCTURE version 2.1 [ 50] and NEWHYBRIDS version 1.1 [ 51] to identify hybrids based on individual microsatellite genotypes in comparison to mitochondrial haplotypes.
We applied two complementary Bayesian clustering algorithms, namely Structure v.2.1 [ 18, 19] and Geneland v.1.0.7 [ 20], to infer population structure (i.e., a number of clusters, K) and to assign individuals (probabilistically) to populations (or clusters) based on individual multilocus genotypes and, for the second algorithm, also on individual spatial origins.
Estimated MAFs in exome sequencing data correlated with those based on individual sample genotyping (Supplementary Figure S1).
These errors cause some differences between the markers detected as significant by pooling and those found significant by interval mapping based on individual selective genotyping.
Concerning P. bocagei and P. hispanica type 1A, these were based on individual multilocus genotype clustering [ 18] and the observation of interspecific matings in nature (R. Ribeiro and M. A. Carretero, pers.
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