In this study, two arrays, based on independent pools of biological replicates, were used for each comparison of interest.
Each PCR was replicated at least three times, based on independent biological samples.
To optimize model performance, we developed 100 replicate models of ecologic niches based on independent random subsamples from available occurrences.
Vertical bars show the standard deviation of the mean based on two independent replicates.
Standard deviations are reported based on four independent replicates.
Total RNA was then extracted from the cells for qRT-PCR based on three independent replicates.
Data show the mean ratio of firefly to Renilla luciferase activity based on three independent replicates.
Based on 15 independent replicates of single MII oocytes, 7,470 genes (10,428 transcripts) were identified in the MII oocytes.
Results are based on three independent replicates with subsequent analysis performed using "The Institute for Genomic Research TIGRR) microarray software suite".
Quantification of meaB expression was conducted using a phosphorimager (STORM 860) and based on four independent replicates.
