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For this purpose, we designed 100 primer pairs based on identified microsatellites loci used for genetic mapping.
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We identified microsatellites and developed primers based on 454 shotgun sequences.
The identified microsatellites were characterized as perfect (mononucleotides to hexanucleotides) and hypervariable Class I (≥20 nucleotides) and Class II (12 20 nucleotides) based on the length of the repeat motifs.
All identified microsatellites with designed primers were condensed into 2322 unique clusters (i.e., unique loci) of which 360 were shown to be polymorphic based on multiple sequence reads with different repeat motifs.
In West Africa, A437G single mutants prevail and three independent ancestry lineages have been identified based on the two microsatellites most proximal to the dhps gene, 0.8 kb and 4.3 kb [ 13].
The analysis based on nine microsatellite markers identified high intrapopulation genetic diversity, evidence of sexual recombination, and geographic differentiation.
Likewise, PCR fingerprint patterns based on M13 microsatellite DNA identified 2 major genotypes among the strains of serotype A. In this group, the VNI strain type was the most common genotype found worldwide (5 ).
FIS values are shown in Additional file 7. Our phylogeographic and population genetic analyses based on eight microsatellite markers identified five principal clusters of populations in the Italian vairone.
Animal pairs were identified based on microsatellite analysis as described previously [35].
Based on 11 nuclear microsatellites, we identified 86 unique genotypes over the two sampling periods and determined levels of genetic structure.
We used two Bayesian methods implemented in the programs STRUCTURE version 2.1 [ 50] and NEWHYBRIDS version 1.1 [ 51] to identify hybrids based on individual microsatellite genotypes in comparison to mitochondrial haplotypes.
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