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Significant pathways were calculated based on hypergeometric analysis; pathways of interest were defined as having a Benjamini and Hochberg corrected P value of < 0.05 (Benjamini and Hochberg 1995).
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P values were calculated based on hypergeometric distribution analysis.
Significantly enriched components defined in Gene Ontology (GO) were determined based on hypergeometric distribution analysis [ 38].
Moreover, based on hypergeometric distribution analysis, the tool is also able to identify a list of significantly-enriched GOs for every possible pair of miRNAs.
Genes associated with the biosynthesis, signalling and response to stimuli by JA, SA, ABA (abscisic acid) and ET phytohormones were significantly overrepresented in the flower bud transcriptome affected by the boll weevil based on hypergeometric distribution analysis (p-values ≤ 0.005).
This incidence represents a significant overrepresentation (P = 1.9 × 10−10, based on hypergeometric distribution analysis) relative to the ca. 26% of the yeast genome that consists of parologous combinations (17).
For cellular pathway analysis, MetaCore is based on hypergeometric distribution, SAM-GSA uses "Maxmean" statistic, and GSEA uses modified Kolmogorov-Smirnov statistic.
Based on hypergeometric distribution, we carried out a function enrichment analysis between the progression-related sub-network and the whole human biological network.
AmiGO database [ 38] was used, and statistical analysis was performed using R program based on hypergeometric distribution followed by Fisher's exact test [ 39].
Significance analysis of GO was performed by the gene enrichment based on hypergeometric distribution finished by Fisher or Chi-sequare test.
Enrichment analysis on metabolic pathways was calculated for each dataset using Fisher exact test based on hypergeometric distribution with a p-value cutoff of 0.1.
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