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On the other hand, the ORAC method is based on hydrogen atom transfer, this may explain the low value of the correlation coefficient between TPC and ORAC.
There are methods, such as ORAC, based on hydrogen atom transfer, in which the antioxidant and the substrate compete for thermally generated peroxyl radicals through decomposition of azo-compounds [19].
However, it has been recommended that assays based on hydrogen atom transfer and electron transfer reaction together should be used to provide a better view of antioxidant activity than a single method [ 17].
In fact, depending on the reaction involved, these assays can roughly be classified into two types: assays based on hydrogen atom transfer reactions and assays based on electron transfer [ 59].
Several antioxidant assays are frequently used to estimate antioxidant capacities in fresh fruits and vegetables, these assays can roughly be classified into two types: assays based on hydrogen atom transfer (HAT) reactions and assays based on electron transfer (ET) [ 20].
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Although definitions based on hydrogen bonding provides some rigor in assigning secondary structure, the standard definition of what constitutes a hydrogen bond is based on the notion of bond energy whose measurement can be imprecise and acutely sensitive even to small differences in the position of nitrogen and carbonyl atoms, especially the carbonyl oxygen positions.
The threshold of distance cut-off is based on hydrogen bonding and van der Waals attractions: (1) a hydrogen bond was defined as having a maximum donor acceptor distance of 3.35 Å and maximum hydrogen-acceptor distance of 2.7 Å. (2) atoms were considered to form van der Waals contacts if the distance between them was ≤ 3.9 Å and the contact had not been defined as a hydrogen bond [ 5].
This assumption is based on the hydrogen atom abstraction mechanism but does not take into account other possible one-electron transfer mechanisms of the N-dealkylation reaction [16].
The efficiency of an antioxidant component to reduce DPPH largely based on a hydrogen atom mechanism while the ABTS assay is based on an electron transfer mechanism with faster reaction kinetics [ 52].
To ensure potent biological activity of the ethanolic extract of Padma 28, its antioxidant capacity was evaluated using the Oxygen Radical Absorbance Capacity (ORAC) assay, which measures the direct capacity of chain-breaking antioxidants based on the hydrogen atom transfer mechanism in a cell-free system.
While scientists cannot directly observe hydrogen molecules on the sun, they searched for a stand-in, the molecule hydroxyl, which contains one hydrogen atom and one oxygen atom.
More suggestions(16)
based on hydrogen adsorption
based on hydrogen fuel
based on hydrogen exchange
based on hydrogen concentration
based on hydrogen stream
based on hydrogen abstraction
based on hydrogen release
based on wave atom
based on hydrogen use
based on hydrogen etching
based on hydrogen permeation
based on hydrogen production
based on hydrogen methane
based on hydrogen sulfide
based on element atom
based on hydrogen peroxide
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Justyna Jupowicz-Kozak
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