Sentence examples for based on human sequences from inspiring English sources
In conclusion, by performing a simple validation experiment, real-time PCR assays based on human sequences, which are easily available, can be applied for analysis of baboon samples.
All proteins are based on human sequences, unless otherwise indicated.
All RNAs are based on human sequences, unless otherwise indicated.
"All RNAs are based on human sequences, unless otherwise indicated" was added to the RNA Preparation section.
Primers based on human sequences were tested for these genes, but no PCR product could be created on sheep DNA.
All primers were from Invitrogen (Paisley, UK) and were designed based on human sequences as summarized in Table 1.
Primers used to amplify AMEL exon 6, previously shown to contain the proline-rich repeat motif present in strepsirrhines, were designed based on human sequence information.
A truncated form of Hsp60, designated as Hsp60c, which lacks a mitochondrial targeting sequence (MTS; amino acids 1 26 based on human sequence), was amplified by PCR and subcloned into the pCGN-HA (a kind gift of Dr. W. Herr, Cold Spring Harbor Laboratory) and pGEX-4T1 (Amersham) vectors, by which the HA-tagged and GST-fused Hsp60c expression plasmids, respectively, were constructed.
On the basis of biochemical in vitro studies Ser-42 and Ser-44 and Thr-143 on cTnI [ 30] and Thr-194, Ser-198, Thr-203 and Thr-284 on cTnT [ 17, 34] are considered the main sites (phosphorylated sites based on human sequence).
As a step toward developing molecular tools for cytokine expression studies in these species, primer pairs for 18 cytokine gene fragments were designed based on human DNA sequences and used to amplify the corresponding genes in Aotus infulatus and Saimiri sciureus genomic DNA samples.
