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In a global ranking based on highest expression levels at day 6, the three predicted hydrophobins encoded by An03g02400, An08g09880 and An03g02360 were at positions one, five and six, respectively.
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We have identified a CSC population in ESFT cell lines and xenografts based on high expression of ALDH.
Based on high expression level and early plaque formation line ARTE10-729 waselecteded for propagation and subsequent analysis.
Comparing the gene expression profile of un-treated and treated melanoma cells implicated Wnt signaling based on high expression of the Wnt antagonist SFRP1 only in sensitive cells, which led us to further explore downstream members of this pathway and to identify activated β-catenin as a feature contributing to drug resistance.
After flow cytometry, blots were gated on ASCs based on high expression of CD38 and CD27 as shown in Figure 4. Alternatively they were gated onto CD3−CD14−CD20−IgD−CD19+IgG+CD38+ cells relaxing the CD38 gate and including cells that showed intermediate expression.
Five miRNA candidates, miR-30c, miR-146a, miR-221, miR-328, and miR-375, were selected from the miRNA screen carried out using the discovery cohort based on high expression levels and high discriminatory capacity.
The first 18 regions (Table 2) were chosen based on high expression levels of adjacent genes in placenta as ascertained from RNA-seq data (Jin et al. unpublished data).
A clear role for TcBBM in the embryogenic process is none-the-less evident based on high expression throughout embryo development as well as in the embryogenic calli but not in the non-embryogenic calli.
Based on high expression of α2 β1 integrin, Collins and colleagues identified PSCs in the basal layer and showed that the α2 β1hintegringrin cells represent ~1% of basal cell population in the human prostate [ 51].
Based on the microarray data analysis of the group of short-term cultured spermatogonia, the following germ cell- and spermatogenesis-associated genes were selected for the real-time PCR analysis: PRM1, PRM2 2, TNP1, CRISP2, ODF1, ODF2, ADAD1, PHF7, SPATA6, TMEM31, MAEL, C9ORF9, and GTSF1, as based on high expression levels (see Tables S3A and S3C).
For gene expression profiling of long-term cultured spermatogonia the following genes, associated with stem/germ cells and spermatogenesis, were selected: PTN, MARCKS, SMAD5, POSTN, ALDH1A2, SRFP1, CUL3, HS6ST2, SOX9, COLEC12, TSPYL1, and RGS4 based on high expression levels (see Tables S3B and C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com