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Plasma membranes and caveolae fraction were isolated from whole lysate by a detergent-free purification protocol based on gradient fractionation, and equal amounts of proteins were analysed by western blotting.
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One category is based on gradient, including finite difference approximation of derivative, adjoint gradient-based methods, and gradient simulator methods.
It is based on gradient descent method.
The parameters are iteratively updated based on gradient descent.
The fractionation/isolation step was based on gradient elution method [ 10] with the Hexane-Ethyl acetate (Fisher Scientific AR Grade, UK) solvent system.
This indicated that a calculation of biodegradation based on isotope fractionation could work in systems like soil columns.
NMD substrates classified based on cell fractionation assays as "nucleus associated" or "cytoplasmic" are all inhibited in the same manner.
The model system used is based on subcellular fractionation of rat brains followed by ligand binding and functional measurements.
A second generation of strategies has studied subcellular compartments based on co-fractionation with marker proteins on density gradients (Andersen et al, 2003; Dunkley et al, 2004; Foster et al, 2006).
The collected fractions were screened for their anticancer property, based on bioassay guided fractionation.
To achieve this goal, we isolated PLSs through a fractionation procedure based on density-gradient centrifugation.
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