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Screening panels based on genotyping platforms, such as OncoCarta?
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The sampling method was based on Hindorff et al. [ 8] and aimed to obtain sample sets of SNPs of equal size to the set of trait-associated SNPs represented on genotyping platforms.
Finally, genotype imputation enables meta-analysis that combines results across studies based on different genotyping platforms [ 37, 38].
Based on genomic position, a total of 107,896 SNP were shared between the 2 platforms; however, based on genotype concordance, only 96,031 SNP had complete concordance at these loci.
For any CN technology based on a genotyping platform (essentially all allelic CN technologies), one might expect genotyping performance to correlate with CN performance.
Animals were used based on genotypes.
We compared the permutation results with results obtained by a sampling strategy based on Hindorff et al. [ 8], which randomly samples genotyping platforms and SNPs from the HapMap II project present in CEU (Caucasian population).
A set of SNPs identified across all samples was filtered to look for those suitable for genotyping platforms such as Illumina with a minimum spacing between SNPs of 50 base-pairs (bp) flanking nucleotides on either side of a SNP.
The SNP assays are based on KASParTM genotyping chemistry and were detected using the Fluidigm dynamic array platform.
Dense consensus genetic maps based on high-throughput genotyping platforms are valuable for making genetic gains in Brassica napus through quantitative trait locus identification, efficient predictive molecular breeding, and map-based gene cloning.
The mass spectrometric SNP genotyping technology based on the Sequenom platform provided an ideal choice for mutation profiling to address these several criteria.
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