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The absence of a universal signature gene has hampered classification of viral genomes based on genomic sequences Recent studies of bacteriophages have identified conserved patterns of oligonucleotides used as genome signatures unique to each genome analyzed that appear to be co-evolving with their hosts [ 25].
Therefore, it is anticipated that the gene catalog presented here will be a better representation of the PST gene complement, than the previous one which was based on genomic sequences alone [ 14].
Primers (Table S1) were designed based on genomic sequences (X03935) of non-glutinous maize using Primer3 [29].
We have tested predictions based on genomic sequences using publicly available EST and exon array data.
The second stage complements the first and involves additional predictions based on genomic sequences.
Nucleotide positions were numbered based on genomic sequences of SMAD3 [GenBank: NC_000015.8] and SMAD4 [GenBank: NC_000018.8].
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These cross-species analyses were based on genomic sequence-based orthologous relationships, downloaded from Ensembl Compara (http://www.ensembl.org).org
As with C. teleta, BLAST searches of the H. robusta trace files on NCBI and Genscan predictions based on genomic sequence of the assembled genome on the JGI website resulted in the identification of two putative Dlx genes.
BLAST searches of the C. teleta trace files on NCBI and Genscan predictions based on genomic sequence of both the assembled genome on the JGI website and of a genomic contig generated manually from trace files resulted in the identification of two putative Dlx genes.
For the human chimpanzee fusion experiment, primers were selected by identifying non-polymorphic primer sequences flanking intron-spanning amplicons that contain at least one single-nucleotide substitution between the two species based on genomic sequence alignment.
An analysis based on genomic sequence can reveal the relationships among strains more accurately, so additional genome analysis is required to resolve the observed discrepancies.
More suggestions(15)
based on gene sequences
based on genomic co-ordinates
based on genomic positions
based on genomic drivers
based on genomic distances
based on genomic data
based on genomic predictions
based on unique sequences
based on genomic relationships
based on human sequences
based on genomic analyses
based on genomic fucosyl-
based on genomic coordinates
based on genomic studies
based on nucleotide sequences
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