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Primers for the detection of BTV 8 were based on genome segment 2 of the virus, the VP2 gene.
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A genome-scale metabolic model, iJM658, was reconstructed based on genome annotation and literature mining.
The circular binary segment algorithm implemented in BioConductor DNAcopy package (http://bioconductor.org) was applied to the log2 ratio data to segment the chromosome into contiguous regions of equal copy number based on a maximal t-statistics with the use of a permutation reference distribution (using coordinates of the SNP based on Genome Build HG-17).
Similar conclusions resulted after a more detailed sequence analysis based on five genome segments (Seg-2, -7, -8, -9 and -10) of BTV-4 isolates from different Mediterranean countries [31].
DIALIGN [ 13] employs local alignment based on segment-to-segment comparison to construct the final alignment.
Phylogenetic analyses based on the small genome segment generated by primers 222/292 provided sufficient information to identify the relationship among the CA24v isolates and the probable origin of AHC strains in circulation in Brazil.
Recently, Matthijnssens et al. (10 ) proposed a classification system based on all 11 genome segments (4, 7, 10 ).
In contrast, the virus Jirandogo, the first arenavirus reported from M. baoulei, in various phylogenies based on its different genome segments belongs to the Lassa virus group (similarly as Gbagroube virus).
However, the recent developments of standard operating procedures for calculating genome-to-genome distances based on high-scoring segment pairs and new computational tools that allow the digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison are significant advances in genomic taxonomy [ 41- 43].
Supplementary Table S1C and D describes the categorization of the CCGG segments based on the genome annotations.
Additionally, each peer stores segments based on the proposed segment distribution scheme to reduce server stress.
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