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This is in contrast to a more typical design in which signatures associated with phenotypes are defined strictly based on genes with expression profiles that match those phenotypes (for example [21]).
All statistics were based on genes with an FPKM > =10.
GO annotation was then inferred using blast2go based on genes with GO entries in the nr database [ 36].
Comparison of results with the Affymetrix based dataset, based on genes with common Unigene ids on both platforms, show a similar pattern of differentially expressed genes.
We used a gene-centric approach based on genes with a high prior probability of association with the traits of interest in NPHSII.
Global biological responses to fasting and to insulin neutralization were further characterized using KEGG pathway matching, based on genes with statistically significant differential expression (FDR < 0.05) and absolute fold-change ≥1.5.
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Its high amino acid similarity with Pdr5p (70%) and significant difference of sequence with SAKL0C11704g and ZYRO0A04114g suggest an evolution based on gene conversion with one of the member of the ZYRO0D11836g, ZYRO0D11858g and ZYRO0D11880g tandem gene array.
The 135 k Brassica Exon Array was validated by quantifying transcriptome differences between leaf and root tissue from a reference Brassica rapa line (R-o-18), and categorisation by Gene Ontologies (GO) based on gene orthology with Arabidopsis thaliana.
A total of 55% (50) of the in silico predicted essential genes agreed with the in vivo essential genes based on gene homology with P. aeruginosa.
For the unsupervised hierarchical clustering of subjects based on gene expression, genes with average expression among all samples <5 FPKM were excluded.
A different strategy is based on gene prediction with statistical models.
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