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Partial gene models were verified based on gene structures, presences of conserved domains and EST (expressed sequence tag) supports.
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We decided to prioritize candidates for sequencing based on gene structure and known mutant phenotypes; ENU mutants are biased towards mutations in genes with longer coding sequences and more exons [12].
Based on gene structure and protein kinase domain sequence divergence, we defined 5 groups; this classification differed from Shiu et al.'s that divided the LKs into 15 subgroups or groups according to phylogenetic results [2].
Based on gene structure, MYB genes have been classified in four different groups [ 12].
Redundant and invalid gene models were verified based on gene structure, intactness of conserved motifs, EST support and synteny analysis.
Type IV secretion systems can be divided into subgroups based on gene structure and evolution (Fig. 2).
Furthermore, based on gene structure data, we inferred a model for the evolution of the GHF5 endoglucanase gene structure in plant-parasitic nematodes.
Based on gene structure and phylogenetic analyses, vertebrate glycine channels are thought to be orthologous to the invertebrate GluCl channels (Vassilatis et al., 1997).
The primer sequences were designed based on gene structure models at the Arabidopsis Information Resource http://www.arabidopsis.org (TAIR) using LightCycler Probe Design Software 2.0 (Roche, Mannheim, Germany) and by setting the primer melting temperature at 60°C.
The duplicate genes in the fish genomes presented a nomenclature challenge and I am very grateful for help from the Zfin nomenclature coordinator [ 62, 63] in choosing the most appropriate names for these genes based on gene structure similarity and synteny.
The 12 MaSOD genes were divided into four groups based on their conserved motifs, which corroborated their classifications based on gene-structure patterns and subcellular localizations.
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