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The extent of gene rearrangement within such an evolutionarily young group offers the opportunity to explore gene dynamics such as tRNA remolding, which appears to have been rife within this family, and to investigate its consequences for phylogenetic analyses based on gene orders.
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Distances between complete genomes can be computed by estimating the number of rearrangements needed to transform one genome into another based on gene order.
However, trees based on gene order, lack resolution as there are very few genome rearrangements observed in nature [19].
Phylogenetic analyses based on gene order (fig. 3 A and B) were ambiguous.
The relative chromosomal location of the genes, based on gene order in N. crassa, is shown in Figure 1.
Based on gene order alone, the most parsimonious position for Xenoturbella is as a basal hemichordate (hypothesis X6, table 1).
Chromosomal clustering methods for co-expressed genes were based on gene order with a background gene list comprising the 13,773 genes represented on the Affymetrix microarray.
The definition of the genomic core based on gene order conservation was demonstrated to be more robust than the simpler approach based only on gene conservation.
These authors found a strong discordance between a phylogeny based on concatenated conserved amino acid sequences and reconstructions based on gene order.
This emphasizes that phylogenetic inferences based on gene order conservation are problematic when the exact evolutionary relationships of the genes remain uninvestigated.
In this way, it was possible to assess the best supported position of Xenoturbella within a phylogeny of the Metazoa based on gene order evidence.
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