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The University of California, Davis, led a National Science Foundation NSFF) supported effort to design, print and validate 22K and 45K oligonucleotide arrays based on gene model predictions from TIGR's osa1 version 3.0 release.
Ortholog assignment between C. elegans and C. briggsae is further improved based on gene model improvement, sequence similarity, and synteny when applying our gene model improvement procedure.
The data for each experiment were step-wise normalised [ 78] and filtered to remove spots with an A-value below 8. Finally the separate data files were median aggregated based on gene model and merged to a single matrix.
The novel variations, plus the known disease-associated variations, are then further classified as intergenic variations, intronic variations, 5′/3′-UTR variations and splice site variations, based on gene model definitions.
An Affymetrix GeneChip whole genome tiling array was designed, and analyzed at the gene and exon level analysis with a total of 524,909 sense strand probes (average of 16 probes per gene), based on gene model predicted transcripts for T. pseudonana, version 3.0 (http://genome.jgi-psf.org/Thaps3/Thaps3.home.html).html
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Two 1573-bp alaccaselaccase genes from L. edodes L54 were cloned based on gene models in the genome sequence.
Oligonucleotide primers were designed based on gene models from the MIPS database (www.arabidopsis.com).
As is a general rule for C. elegans, the interpretation of our previous studies was based on gene models and functional annotations present in the nematode-specific database WormBase at the time [45].
(XLSX 44 kb) Additional file 18: Candidate CRNs of Pl. halstedii, predictions based on gene models.
(XLSX 38 kb) Additional file 20: Candidate RxLR-like effectors of Pl. halstedii, predictions based on gene models.
The majority of the methods used to predict genes are based on gene models reconstructed solely from mRNA and EST evidence.
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based on gene co-expression
based on gene set
based on gene order
based on gene deletion
based on gene expression
based on gene percentage
based on gene duplication
based on gene copy
based on gene protein
based on gene structure
based on gene essentiality
based on gene homology
based on gene orientation
based on gene annotation
based on gene architecture
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