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Huson and Steel have demonstrated the superiority of Dollo parsimony over distance-based methods for the deduction of phylogenetic trees based on gene content [ 87].
Here, we have estimated the phylogenetic relationships of the genus using two new approaches: One based on a Mycobacterium-specific amino acid substitution matrix inferred from the protein-based alignments of 19 genomes and the other based on gene content dissimilarity.
Successfully predicting the capacity for natural transformation based on gene content alone is problematic due to species-to-species variation in competence systems [68].
Based on gene content, some of the smaller elements likely are remnants of ancestral genetic elements that have undergone reductive evolution.
A cladistic analysis based on gene content of isolates allowed strains circulating in each infected family to be defined (Fig 1, 2, 3).
For the generation of a phylogenetic network using the neighbour-net algorithm [56], pairwise genome distances were computed based on gene content using SplitsTree4 [55].
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Results may be downloaded as phylogenetic matrices for subsequent analysis, including the construction of whole-genome phylogenies based on gene-content data.http://rodeo.med.harvard.edu/tools/roundup.edu/tools/roundup
Methods that are not based on gene-content do not place such a strong emphasis on shared genes such as those involved in methanogenesis, perhaps for this reason instead of grouping with the other methanogens in the supertree M. kandleri is found in a clade with Arc.
More than 30 different KIR haplotypes exist based solely on gene content (10).
The region containing EXT repeats at chromosome extremities can be differentiated based on gene composition, GC content, recombination sites density and chromosome landscape.
Regarding the latter, we identified 1,478 genes that were universally present in all 181 S. aureus and 143 S. epidermidis isolates (fig. 2 A) corresponding to approximately 52% of the full gene complement of an S. aureus genome, and 56% of the full gene complement of an S. epidermidis genome, based on average gene content.
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