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Gene specific primers were designed based on gene annotations to amplify full-length coding sequences of CquiOR2 (XM_001864509/CPIJ014392) and CquiOR10 (XM_001844036/CPIJ002479). Antennae, maxillary palps, proboscis, legs and bodies (thorax and abdomen without head/legs) cDNAs from female adult mosquitoes (one-to-seven-day-old) were used as templates for tissue-specificity study.
We annotated Dsgs that transposed into unique loci, based on gene annotations provided by MaizeGDB [ 16]. 444 Dsgs had inserted inside or within ±1 kb of genes.
Candidates for pi66 t) were predicted based on gene annotations provided by BLASTN (www.ncbi.nlm.nih.gov/BLAST), RiceGAAS (ricegaas.dna.affrc.go.jp) and FGENSH (www.softberry.com) software.
In conclusion, the P. pastoris GSMM was comprehensively improved based on gene annotations from different databases and literatures (Additional file 1).
Based on gene annotations and transcript abundance data we identified candidate genes.
The given examples illustrate that SNP oligomers that are mapped to different genomic locations may lead to completely functionally different inferences based on gene annotations.
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There were two copies of Ir40a and Ir93a in D. sechellia and D. simulans but only one copy in D. melanogaster based on gene annotation.
The main limitation of this approach is that it is primarily based on gene annotation, and that it is therefore biased towards selecting better annotated genes.
For each section, the transcripts mapping back to these spots were added together based on gene annotation and averaged over the ten spots.
Based on gene annotation database, Singular Enrichment Analysis (SEA) was conducted in different comparison groups using the agriGO tool.
We organized functional gene subsets based on gene annotation and pathway information.
More suggestions(13)
based on gene organization
based on literature annotations
based on gene insertions/deletions
based on contig annotations
based on gene IDs
based on gene diversities
based on gene models
based on gene studies
based on gene pairs
based on gene lists
based on gene predictions
based on gene names
based on gene ontologies
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