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The ability to globally screen for PTMs based on gel shift is a major advantage of the 2D DIGE approach.
The effect of AICAR on transcriptional activation was mediated by an overlapping GATA/EBox binding site at −495 within the PGC-1α promoter based on gel shift analyses that revealed increases in GATA/EBox DNA binding.
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Based on gel filtration analysis, MakA is undoubtedly monomeric in solution and pore-forming activity has not been detected.
The selection is based on a gel shift assay of a messenger RNA (mRNA)–protein fusion product that is synthesized in a cell-free translation system using an mRNA display method.
This was based on reconstitution and gel shift assays indicating that Mia40 can be with all three of its cysteine pairs completely reduced both in vitro and in vivo.
However, based on mobility shift experiments in denaturing-PAGE gels lacking reducing agents, we can find no evidence for such structures in our transfected cell-lines (data not shown).
For instance, let the selected shift based on the shift specification algorithm be night shift.
The CO2-sensitivity of the gel system was demonstrated using transparent tubes and the gel strength and gelation time were evaluated based on a gel strength code method.
Quantitative analysis was based on average gels created from three gel replicates.
We do not provide data based on shift length.
The algorithm is based on the mean shift algorithm[17].
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