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Three primer pairs with fragment lengths of 194 bp, 163 bp and 389 bp, respectively, were compared, and based on gel quantification analysis by ImageJ 1.46r software, primer pair No. 1 (P1 and P2) was selected for use in conventional PCR and nanoPCR assays (data not shown).
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It is worth mentioning that loading based on only quantification on gel would have led to overloading the Solexa and would have produced many but unreliable sequences.
Based on quantification by 2-D gel electrophoresis, legumin levels were raised by 3-fold, to 17% of total protein, while albumin-2 was elevated by 10-fold, to 1.2% of total protein.
Patient studies with divergent quantitative diagnosis based on the quantification method used.
Based on the quantification, the superiors reward or punish the quantified key tasks.
Similarly, estimates based on TMT quantification were poorly correlated with estimates based on 100% chemical acetylation (Appendix Fig S2B).
The quantification of mRNA levels was based on the relative quantification method (2-ΔΔCt) [ 24].
Quantitative analysis was based on average gels created from three gel replicates.
As a result, a growing number of significant statements based on quantification can now be made.
Fig. 6 Divergent quantitative diagnosis based on method of quantification.
The method is based on quantification of T-cell receptor excision circles (TRECs) extracted from DBSS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com