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This article addresses inherent problems in structuring a nanoEHS research strategy based on the goal of predicting outcomes directly from nanomaterial properties, and proposes a framework for organizing data and designing integrated experiments based on functional assays (FAs).
Based on functional assays, we classified the EVP (T6SS) proteins into three groups: (1) eleven intracellular apparatus (non-secreted) proteins, (2) three secreted proteins, and (3) two proteins which are not required for the T6SS-dependent secretion.
This evidence is based on functional assays, as we could not detect accumulation of ROS in the treated cells.
The first evidence of nestin-/CD133-positive cells in rhabdomyosarcoma samples and cell lines (Fig. 3a) was followed by verification of their tumorigenic potential based on functional assays.
Nevertheless this definition is mainly based on functional assays and methodological artefacts have hampered the identification of these organelles in TEM.
Our strategy is based on functional assays developed for the identification of TICs (Vescovi et al, 2006; Fael Al-Mayhani et al 2009).
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Significant progress has been made in our understanding of changes in T cell responses during immunotherapy, but existing work has largely been based on functional T cell assays.
Recent results, including our own investigation based on functional or DNA binding assays in cell systems or cell extracts, established that maximal transactivation requires adjacent dimer binding sites [20] [26].
Recent review articles have focused on new methods and new signal amplification based on functional nanomaterials and enzymes in the DNA and RNA assays.
Classifications are based on functional mobility.
The IPC oversaw classification based on functional disability.
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based on immunoenzymatic assays
based on multiple assays
based on proteomic assays
based on immunoluminometric assays
based on immunochromatographic assays
based on immunosorbent assays
based on enzymatic assays
based on standardized assays
based on immobilized assays
based on phenotypic assays
based on traditional assays
based on serological assays
based on experimental assays
based on triplicate assays
based on functional analysis
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