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Two calibration time points based on fossil records, O. latipes T.
Critics, including The Skeptical Environmentalist author, Bjørn Lomborg, have argued that because such figures rely on so many estimates of the number of underlying species and the past rate of extinctions based on fossil records of marine animals, the huge margins for error make these figures too unreliable to form the basis of expensive conservation actions.
In opposition to this, other authors argue that it is impossible to identify this species based on fossil records, because they only allow for examination of shell features.
There is only one population of this species at Chittenango Falls State Park in central New York, even though it was believed to have a broader range previously; based on fossil records, some authors argue that the distribution of this species may have been much wider in the past.
Most of them are based on fossil records of species separation, namely the New World/Old World monkeys split or the Hominid split.
These Mediterranean populations may be relicts of a wider Pleistocene distribution, when, based on fossil records, L. saxatilis lived possibly as far south as Gibraltar and the coast of Morocco; alternatively, they are the result of more recent introductions [16].
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The divergence of the maize, rice, and wheat lineages based on fossil record is estimated at 50 70 Ma (Wolfe et al. 1989).
Molecular clock analyses on complete mitochondrial genomes from C. bactrianus ferus and Lama pacos suggested that the two tribes diverged from their common ancestor about 25 million years ago, much earlier than what were suggested based on fossil record.
Among the most significant advances common to these new methods is a departure from the molecular clock assumption and the use of time constraints at multiple nodes for rate calibration, usually based on fossil record.
Past work, based on fossil record, morphology of extant taxa, and phylogeography (Tibet origin) suggested that the major burst of radiation in the Triculinae occurred late Miocene (12 5 Ma) [ 15].
For example, recent studies have assumed constant rate (strict) molecular clock models using tightly bound (fixed) calibrations based on fossils records or biogeographic events from the extant hosts [ 10, 17- 19].
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