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Live cells were gated based on forward scatter and side scatter and/or by propidium iodide dye exclusion.
Cells were gated or sorted based on forward scatter (FSC) and side scatter (SSC) and positive fluorescent surface markers.
The lymphocytes were first gated based on forward scatter (FSC) versus side scatter (SSC) with the dead cells excluded using the violet live/dead stain.
Cells were first gated on CD3 positive events, followed by a gate on resting lymphocytes and proliferative blasts based on forward scatter (FSC) vs. side scatter (SSC) parameters.
Sorting was based on forward scatter and side scatter properties (FSC/SSC) and surface marker-positive fluorescent gates; CD3 for T lymphocytes; CD19 for B lymphocytes; CD14 in combination with CD16b to distinguish monocytes and neutrophils.
Finally, the increase in parasite numbers was accompanied by predictable changes in cell size based on forward scatter analysis by flow cytometry analysis with large parasites present in S phase and mitosis (post-release 2 4 h) becoming smaller as daughters resolve their mother at the end of cytokinesis (post-release 5 6 h) (not shown, see [25]).
Similar(34)
The samples were gated, based on forward scattering properties and red autofluorescence of Chla, to eliminate signals from NPs, debris and other contaminants.
Cell debris was electronically gated out, based on forward light scatter.
The CSD is monitored by using an on-line CSD measuring instrument which is based on forward light scattering.
The remaining cells were identified as lymphocytes or granulocytes based on forward and 90° scatter.
Cancer cell populations were gated based on forward and side scatter variables.
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