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In such kind of situations, in order to avoid unnecessary interface shifts, a hysteresis mechanism based on forward and backward protection is one of candidates to compensate for it.
Two strategies are presented, based on forward and backward sequential algorithms.
Neutrophils and mononuclear cells were discriminated based on forward and sideward scatter46,47 and live and dead cells were discriminated with DAPI staining.
(d) Primitive and definitive erythroids were sorted from E15.5 control and Nkx2 5IRES-Cre/+; R26YFPR/+ embryos based oNkx2 5IRES-Cre/+de scatteR26YFPR/+embryosels).
The sensitivity and uncertainty computations were performed by means of the SUSD3D code based on forward and adjoint-flux first-order perturbation theory.
The remaining cells were identified as lymphocytes or granulocytes based on forward and 90° scatter.
Cancer cell populations were gated based on forward and side scatter variables.
But keep in mind that this low multiple is based on forward and aggressive forecasts of corporate profits.
Histograms represent cells gated for lymphocyte phenotype based on forward and side scatter data.
Dead cells were excluded from analysis based on forward and side scatter profiles.
Individual cell populations were separately evaluated by gating based on forward and side scatter and/or expression of surface markers.
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