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Filtered genes were then compared based on fold expression difference and t-test (p-value of 0.05).
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Comparing the expression patterns using all the 54,675 probes representing all the genes in the human genome or using 45 probes selected based on fold of expression changes induced by E2, action of SWT is similar to E2 in particular when focusing on the 45-probe "estrogen-responsive genes".
Two-way comparative analyses based on fold differences of expression values were performed on normalized and rescaled gene expression data derived from each EOC cell line.
Based on fold-change expression, genes that were differentially expressed between the two repeated microarray experiments were subjected to a scatter-plot analysis.
Then, 25 miRNAs were selected based on fold changes and expression level for further qRT-PCR analyses.
Candidate targets were selected based on fold differences in expression between the two genotypes at day 7 of SE induction with no consideration of target gene identity (Tables 2, 3; Additional file 1).
For both the Colorectal DSA and the Plus2.0 complete microarray data, following detection filtering, probesets were further filtered based on fold change in expression and a statistical filter in the case of both HCT116 parental and 5-FU resistant cell line data.
Table 1 shows a list of IFN-γ regulated genes whose expression are affected by H37Rv exosome treatment and includes the genes most altered by exosome treatment based on fold differences in gene expression.
Without any p-value adjustment for multiple tests or filtering based on fold change in the expression, 2.4% and 2.1% of the genes were differentially expressed in COX + and COX++ lines, respectively, compared with the wild type.
We rank-ordered genes based on fold change in gene expression during normal mesoderm differentiation (day 4 control versus day 2) and compared normal and Brg1-deleted mesoderm differentiation.
Standard k-means clustering using 100 iterations and k = 7 (k>8 resulted in progressive separation of genes into redundant clusters) generated gene lists that were highly similar to those generated by the change in fold expression (results based on fold change are shown in Fig. 4).
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