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Exact(5)
Subsequent analysis based on fold differences identified a total of 124 genes which differed at least two-fold in expression level (compared to the median expression level) in at least three out of the 14 patients.
Table 1 shows a list of IFN-γ regulated genes whose expression are affected by H37Rv exosome treatment and includes the genes most altered by exosome treatment based on fold differences in gene expression.
Two-way comparative analyses based on fold differences of expression values were performed on normalized and rescaled gene expression data derived from each EOC cell line.
Candidate targets were selected based on fold differences in expression between the two genotypes at day 7 of SE induction with no consideration of target gene identity (Tables 2, 3; Additional file 1).
Two candidate protein biomarkers were arbitrarily chosen based on fold differences (Table 1), putative roles in the pathogenesis of prostatitis, and the availability of assay reagents for verification using the gold standard in protein quantification, the enzyme-linked immunosorbent assay (ELISA).
Similar(55)
The WHO criteria for reporting NI assay data for influenza viruses (19 ) are based on fold difference between IC50 values of the test virus and a reference IC50 value (such as median IC50); different criteria are set for seasonal type A and type B viruses.
We performed two-way comparison analysis based on fold-differences using the expression values which contained at least one high reliability score (or P call) per probe set for each EOC cell line.
It is important to note that the PAC algorithm, detailed under Materials and Methods, is in essence a simple formula that predicts outlier exons based on fold change differences between measured and predicted exon expression levels.
Filtered genes were then compared based on fold expression difference and t-test (p-value of 0.05).
In this example, we did not limit our search based on fold change or q value, and chose the default options for the layout of the results page (display expression differences as fold change rather than log2 of fold change, and ordered genes based on significance of gene expression differences (q value) rather than gene symbol).
Data was analysed based on empirical fold differences (student's T-test) as well as number of tumours showing greater than 2 fold up- or down-regulation (Pearson's chi square).
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