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Although there have been suggestions of more than one Cse4 nucleosome per budding yeast centromere based on fluorescent microscopy (Coffman et al., 2011; Lawrimore et al., 2011), more recent evidence confirms that there is only one particle per centromere (Henikoff and Henikoff, 2012; Shivaraju et al., 2012; Haase et al., 2013).
Finally, in order to investigate membrane alterations associated with squalamine action, we have used an assay based on fluorescent microscopy (Live/Dead BacLight, Molecular Probes) (Figure 5).
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While several models proposed mechanisms of force generation by actin polymerization (see [ 48] and references therein), recent experiments based on fluorescent speckle microscopy demonstrate that actin does not directly push the membrane outwards.
In order to supplement the structural information obtained by cryo-ET with the functional state of the molecules involved based on fluorescent labeling we developed a method of correlating light microscopy and cryo-ET.
Median Fluorescent Intensity; The Lyme multiplex assay is based on fluorescent detection technology.
The fluorescent photo-switching systems were prepared based on fluorescent benzimidazole and photochromic naphthopyran.
The wells were scored based on fluorescent intensity of GFP-Ipr1.
Visualization is based on fluorescent and phase-contrast imaging.
The photograph was taken on fluorescent microscopy.
Histopathological diagnosis was based on light microscopy with HE staining.
Lysosome imaging and accurate measurement of pH in MCF-7 cells have been successfully conducted based on this nanosensor via fluorescent microscopy and flow cytometry.
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