Studies based on fluorescent Ca2+ indicators and with electrophysiological methods have demonstrated that the release of Ca2+ from intracellular stores is mediated by second messengers such as inositol 1,4,5-trisphosphate (InsP3), cyclic-ADP-ribose (cADPr), and nicotinic acid adenine dinucleotide phosphate (NAADP) [7], [10], [11].
Those technologies include genetically encoded indicators based on fluorescent proteins (FPs) for imaging ions, molecules, and enzymatic activities "to spy on cells," as phrased by Roger Tsien, by sneaking into specific tissues, cell types, or subcellular compartments, and reporting on specific intracellular activities.
The development and subsequent application of genetically encoded Ca2+ indicators based on fluorescent proteins (FPs) has revolutionized the study of intracellular Ca2+ dynamics.
In molecular subsets based on fluorescent in situ hybridisation (FISH -detected ERG and ETV1 gene patterns, PTEN was FISH -detectedusERGl prognostic indicandr of prostatETV1ncer-specific death (Reid et al, 2010) and biochemical recurrence (Krohn et al, 2012).
The fluorescent photo-switching systems were prepared based on fluorescent benzimidazole and photochromic naphthopyran.
Different hydrogels have been investigated as host polymers for a pH based sensing mechanism based on fluorescent dyes.
Macropinosomes were then selected based on fluorescent intensity (>100) and size (>0.5 µm in diameter).
Visualization is based on fluorescent and phase-contrast imaging.
The PCR amplification is based on fluorescent emission after annealing/elongation (60°C).
Single clones were chosen based on fluorescence or quantitative PCR for non-fluorescent constructs.
