Despite continual effort in using PAD with separation techniques, the current instrumentation of choice is MS combined with liquid chromatography based on fluorescent detection or capillary electrophoresis-based laser-induced fluorescent detection (section 8.1).
In a pilot assay of Hoechst 33342, a rapid assay based on fluorescent detection of compacted chromatin in apoptotic cells, MGC-803 cells were incubated with 50 μg/mL of EAC-B by 0, 2, 8 h.
In this approach, methylation levels are determined based on fluorescent signal detection during MSP and melting profiles during HRM.
In our previous work, rapid detection methodologies have been established based on fluorescent nanobiosensors for simultaneous separation and detection of multiple foodborne pathogenic bacteria.
Detection was based on fluorescent secondary antibody binding and quantified using a Li-Cor Odyssey infrared fluorescent detection system (Li-Cor, Lincoln, NE).
All other conditions were the same as those described in Fig. 2. In summary, a simple, portable detection method based on fluorescent BSA-GNPs probe that allows rapid, on-site, real-time detection of Hg2+ has been developed.
Moreover, the electrochemical strategies for the detection of the amplicon showed to be more sensitive compared with Q-PCR strategies based on fluorescent labels such as TaqMan probes.
The fluorescent photo-switching systems were prepared based on fluorescent benzimidazole and photochromic naphthopyran.
Visualization is based on fluorescent and phase-contrast imaging.
The degree of platelet adhesion or 'cloaking' was measured based on the fluorescent detection of labelled platelets on the surface of cancer cells post incubation, while platelet activation was measured based on the fluorescent detection of platelet surface P-selectin.
TRAP assays have been based on the fluorescent detection of captured proteins [7], [9], [27], lipids [7], [9], [29] or glycoconjugates [9], or even of GFP-tagged MHC class I molecule [30], but this latter approach, which would require the generation of individual GFP-tagged versions for every single MHC molecule being studied, would be rather difficult to generalize.
