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9– 13 Detection modalities are based on fluorescence microscopic cytology, 8– 11 fluorescence spectrophotometry 12 and flow cytometry.
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Gates were based on fluorescence minus one controls.
: accumulation in hippocampal pyramidal neurons based on fluorescence imaging measurements.
The automatically generated ground truth was based on fluorescence data, using standard segmentation functions in CellProfiler.
FITC beads were gated based on fluorescence.
As fluorescence intensity depends on the microenvironment, studies based on fluorescence are usually qualitative in nature.
Among new methods developed are those based on fluorescence, fluorescence resonance energy transfer (FRET), and bioluminescence.
Finally, slides were analyzed and evaluated by the average of staining intensity in 400× magnification on fluorescence microscopic examination by Olympus BX-URA2.
Intrapulmonary cell trapping after CV BMSC injection was further evident on fluorescence microscopic images of formalin-fixed, paraffin-embedded lung slices.
In other systems such assays are based on the microscopic monitoring of fluorescence changes resulting from the degradation of a reporter protein, termed degron, which is fused to GFP.
There was no evidence for the formation of a PA14 biofilm on the surface of the Arabidopsis leaves based on careful microscopic analysis of P. aeruginosa expressing a green fluorescence protein (GFP) in this submerged seedling infection system (data not shown).
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