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DOI: http://dx.doi.org/10.7554/eLife.03427.006 In the subsequent 'detection phase', a visible wavelength laser scanned the domain and an iterative threshold-crossing algorithm analyzed the image to then draw regions of interest (ROIs) for putative cell targets based on fluorescence brightness.
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We report two-photon molecular brightness spectra for a diverse set of organic and genetically encoded probes with an automated spectroscopic system based on fluorescence correlation spectroscopy.
: accumulation in hippocampal pyramidal neurons based on fluorescence imaging measurements.
The automatically generated ground truth was based on fluorescence data, using standard segmentation functions in CellProfiler.
All kinetic parameters were determined based on fluorescence increase in the linear time range (typically 10 15 min).
Quantitative analysis of biochemical microenvironment using spectroscopic techniques based on fluorescence, second harmonic, Raman signals will be covered.
We have developed an assay for the assembly of FtsZ based on fluorescence resonance energy transfer (FRET).
FITC beads were gated based on fluorescence.
As fluorescence intensity depends on the microenvironment, studies based on fluorescence are usually qualitative in nature.
Intensity-based methods track 3D faces based on the brightness constraint, assuming constant intensity of the face across adjacent frames.
This makes deciding the size of the oligomers based on molecular brightness data very challenging.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com