Several disadvantages of this assay led us to design alternative tools based on flow cytometry analysis.
We created a mixed culture of two fluorescently labeled yeast species with approximately equal number of cells from both species based on flow cytometry.
Based on flow cytometry of propidium-iodide stained nuclei5, the T. propidium-iodide stainedously beenuclei5athe T. be about 160 Mb, or 15% larger than that of A. thaliana.
S. cerevisiae (mCherry) and C. albicans (GFP) were grown in YPD liquid culture overnight at 30 °C to OD600 = 0.5, diluted to OD600 = 0.1, and combined in approximately equal number of cells based on flow cytometry.
Tumors were collected on day 7 post transplantation and the ratio between each clone (GFP+Thy1.1− or GFP+Thy1.1+) was determined based on flow cytometry analysis and was normalized based on the initial ratio measured on the day of tumor inoculation.
On the other hand, relatively unexplored, complementary technique based on flow cytometry has not been standardized till date, particularly due to the lack of a proper morphological scoring scale.
To this end, 3600 tests were performed on 1800 aliquots taken from 40 goat milk samples using a combined instrument set-up based on flow cytometry for SCC and Fourier transform infrared analysis for fat, total protein, lactose, total solids, and freezing point determinations.
Herein, detailed protocols for autophagy monitoring methods based on flow cytometry are provided using the GFP-LC3-overexpressing CHO DG44 host cell line or MDC-like molecules in rCHO cells grown as an adherent culture with serum-containing medium or suspension culture with serum-free medium.
Figure 2b shows the phenotype of the subsets based on flow cytometry analysis.
We hypothesised that the fluorescent NET substrate ASP+ could allow determination of hNET function based on flow cytometry.
Recent developments in high throughput screening technologies comprise throughputs of 106-108 based on flow cytometry (Ruff et al. 2012; Tu et al. 2011) and microfluidics (Fallah-Araghi et al. 2012; Kintses et al. 2012).
