Transfection efficiency was routinely above 50% in these conditions, based on flow cytometry of cells transfected with a GFP expressing plasmid.
Based on flow cytometry to measure cell-cycle distribution, these conditions achieved an adequate level of cell synchrony.
The two prior estimates for physical genome size were both based on flow cytometry and muscle cells, but differed substantially.
* HLA genotype was not known prior to tumor resection, phenotype based on flow cytometry of vim+ cells with mouse monoclonal antibodies (Table 1).
These observations also suggest that the non-SP cells acquire SP function or that the gating cells solely based on flow cytometry do not allow absolute separation of these cells.
The final preparation of endometrial CD14+ cells had a purity of 90 95% based on flow cytometry while blood CD14+ cells were 98 99% pure (Figure 1).
Consistent with the activation of the miR-210-ISCU1/2 axin in vascular endothelial cells, based on flow cytometric analysis, such PCNA up-regulation corresponded specifically to PECAM+ pulmonary vascular endothelial cells (Fig 3H).
Therefore, tentative diagnosis of malignant lymphoma was made based on flow cytometry of bone marrow cells before the final diagnosis by lymph node biopsy in about 50%% of LAHS patients.
The purity of NK cell population was found to be greater than 90% based on flow cytometric analysis of anti-CD16 antibody stained cells.
