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In this study, we compared RIMS-based drqPCR with classical quantifications based on external standard curves and the "comparative Ct method".
Calibration of milk and kidney samples was based on external standard, due to variability of tobramycin response in these matrices.
All concentration calculations were based on external standard samples.
The data of the six 28-samplestandard curves were used to generate 61,236 and 244,944 different quantifications by the 2ΔΔCq-approach and based on external standard curves, respectively.
Since the differences were small as compared with errors of analysis, the data obtained (Table 2) were based on external standard calibration.
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The amount of Evans blue was computed based on external standards in the same solvent (1 20 µg/ml) and expressed as per gram of tissue.
All isotope data has a 2-sigma age error of 0.1 0.3 Ma and δDwax and δ18Osc represent pooled precisions of 0.5% based on external isotope standards and analytical errors, respectively.
Analysis is performed using LC-MS/MS operating in multiple reaction monitoring mode and Skyline is used to quantify the metabolites based on external calibration curves (standards from Avanti Polar Lipids) with the use of d7-S1P an an internal standard.
The metabolite absolute quantification is based on external calibration with commercial standards, and is validated by a parallel 1D proton NMR analysis.
"It's a political one, because China cannot be seen making a decision based on external pressure".
A hybrid approach was therefore selected, using a standard randomised phase II design, and using Bayesian techniques to incorporate subjective clinician estimates of treatment effect based on external evidence.
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