Here we present an approach that uses the power of quantitative genomics in revealing the most promising candidate genes for simple potato quality traits based on expression variation by implementing a pooling strategy.
Differential effects of rearing condition were not apparent from clustering of samples based on gene expression variation.
We have previously shown organization of genes into separate modules based on their expression variation in yeast [ 10, 12].
This clustering was based on the gene expression variations among RI lines, the basis of which might arise because of functional differences between alleles arising from sequence variations, genetic background, or both.
Two candidate genes, LOC_Os07g41180 and LOC_Os07g41200, encoding RNA-binding protein and unknown expressed protein respectively, were selected based on sequence variations and transcriptional expression and to be further testified by complementation test.
To get a list of statistically significant genes, we combined the two methods commonly in use in most microarray articles: we applied sequentially a cutoff based on the gene expression variation and a cutoff based on the reproducibility of the measurement of this variation [51].
In this study, we identified genes involved in the transcriptional response to DNA damage based on variation in expression upon exposure to two representative DNA damaging agents, methylmethane sulfonate (MMS) and ionizing gamma radiation (γ-ray), over three orders of magnitude of agent dose.
Breast cancer has been successfully classified using microarrays into clinically relevant subgroups based on variations in gene expression patterns.
Recent progress in medical science has led to a sophisticated classification of breast carcinomas, based on variations in gene expression patterns derived from cDNA microarrays.
Two candidate genes were selected based on sequence variation and expression difference between two parents, which facilitated further QTL cloning and molecular breeding in super rice.
